Assessment of intercentre reproducibility in detection of bladder epithelial cells using a novel cell harvesting device


Session type:

Bensita .M.V Thottakam1, Ghulam Nabi2, Satchi Swami3, Linda Gordon4, Mary McKean5, Alex Wilson6, Sian Chilcott7
1Cytosystems Ltd, Aberdeen, United Kingdom,2University Of Dundee, Dundee, United Kingdom,3NHS Grampian, Aberdeen, United Kingdom,4NHS Grampian, Aberdeen, United Kingdom,5NHS Grampian, Aberdeen, United Kingdom,6Robert Gordon University, Aberdeen, United Kingdom,7Addenbrookes Hospital NHS Trust, Cambridge, United Kingdom


Urinary cytology for the early detection of bladder cancer is associated with high inter-observer variations between centres. The objective of this study was to investigate the transferability of slides prepared using a novel cell harvesting device (CHD) and centrifugation (CF)  between two centres and to determine the accuracy and reproducibility of cell counting between devices and centrifugation.  


50 ml of urine was collected from 34 patients presenting to Urology outpatient clinics for investigation of bladder cancer at two different locations, Aberdeen Royal Infirmary (19 patients) and Addenbrookes Hospital, Cambridge (15 patients). All urine samples were collected, processed and prepared in the same way within 4 hours of void.  Each sample was split into 2x 25ml aliquots, one aliquot was subjected to routine centrifugation at 2500 rpm for 10 minutes in each centre, and the other half passed through an identical novel cell harvesting device x 6 minutes in each centre. Thinprep slides were prepared using a Thinprep 2000 processor and PAP stain applied at each centre for CF specimens, while all CHD urines were prepared and stained in Aberdeen.   Total cell counts on each slide from both centres were undertaken using a standardised high power field microscope and an expert independent cytopathologist based in Aberdeen. 


There were no significant differences in total cell counts within or between the two centres (p-Value= 0.931) either using a CHD or routine centrifugation.  There was consistent evidence of less cellular debris in slides prepared using the CHD.


The early application of a CHD in a routine Cytopathology laboratory at two centres has confirmed the translational ability of the equipment. Further work is ongoing to establish its place in a laboratory setting for central processing.