Assessment of Y-H2AX and 53Bp1 Foci in peripheral Blood  Lymphocytes to predict subclinical Hematoxicity and Response in Somatostatin Receptor-Targetad Radionuclide Therapy for Advanced Gastroenter.


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Sarika Mehta1
1nil

Abstract

Background

We amied to characterize  Y-H2AX and 53BP  1 Foci formation in patients receving Somatostain  receptor-targeted Radioligand Therapy. and explored its role for predicting treament -Related hematotoxicity and treatment response.

Method

A prospective analysis of double-strand break (DSB) Markers was perfor tumorsmed in 21 patients with Advanced gastroenterropancreatic -neuroendocrine tumors. Y-H2AX and 53Bp1 foci formation were evaluated in peripheral blood lymphocytes  (PBLs) at baseline+1h and +24h after administration of 7.4 GBq (177 lu) LU-DoTA-TATE PET/CT before enrollement and after 2 cycles of PRRT accourding to the volumetric modification of RECIST.1,

Results

DSB Marker kinetics were heterogenous among patients . subclinical hematoxicity was associated with Y-H2AX and 53BP1  Foci formation (e.g. change in Y-H2AX cells between baseline and +1h (r= -0.6080,P=0.0045) patient showing early development of new Metatases had less Y-H2AX (P=0.0125) and less 53BP1 foci per cell at +1h (P=0. M0289) and demonstrated a distinct Kinetic pattern with an absence of DSB  Marker decrease at +24h (Y-H2AX ;P=0.0025;53BP1;P=0.0008)

Conclusion

Assessment of Y-H2AX  and 53BP1 foci formation in  PBLS of patients receiving  radioligand Therapy.

Impact statement

Thus with the help of Y-H2AX and 53BP1  foci  formation were evaluted in  peripheral blood lymphocytes (PBLS) at baseline  +1h and +24h after administration of 7.4 GBq (177 Lu) Lu-DOTA-TATE PET/CT before enrollement and after 2 cycles of PRRT accourding to the volumetri modificati hadon of RECIST.1and .     


              DSB Marker kinetics were heterogenous among patients subclinical hematotoxicity was associated with Y-H2AX and 53BP1 Foci formation (e.g.change inplatelete count  vs change in Y-H2Ax cells between baseline and +1h (r=-0.6080,P=0.0045) patient showing early developing of new Metatases  had less Y-H2AX (p=0.0289) and demnstrated a distinct kinetic pattern. with an absence of DSB marker decrease art +24h (Y-H2AX:P=0.0025:53BP1:p=0.0008)                                                                                                                                                                                                                                                                                                                                                                                                                             Assessment of Y-H2AX  and 53BP1 foci formation in PBLS of patients receiving radioliement  Therapy.