Association of Helicobacter pylori vacA genotype with gastric cancer in northern Iran


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Farhad Mashayekhi, Zivar Salehi, Rasoul Zahmatkesh

University of Guilan, Rasht, Iran

Abstract

Proffered paper presentation

Infection of the gastric mucosa with H. pylori results in a number of disease outcomes including gastritis, which precedes the development of peptic ulcer disease (PUD), gastric cancer, and lymphomas of the MLAT (mucosa associated lymphoid tissue lymphoma). The vacuolating cytotoxin vacA and cag pathogenicity island (cag PAI) are two identified virulence factors that are considered to have an important role in the pathogenesis of H. pylori infection.

The aim of this study was to determine the prevalence of the vacA gene among H. pylori isolates and its relationship with gastric cancer in 54 Iranian patients. Of the 32 glmM PCR-positive biopsy specimens, 28 (87.5%) had the vacA signal sequence genotype s1, and 4 (12.5%) had subtype s2. vacA mid- region analysis revealed that 12 (37.5%) were vacA m1 and 20 (62.5%) were m2. In conclusion, these results show that the vacA s1m2 positive strains were predominant in this population of patients with gastric cancer. Thus, the detection of vacA, virulence marker may be used to help the prevention of H. pylori in Iran.