Circulating microRNAs as non-invasive biomarkers for breast cancer
Session type: Poster / e-Poster / Silent Theatre session
Development of blood based tests for monitoring breast cancer is of great interest. As circulating microRNAs (miRNA) have emerged as a useful non-invasive biomarker in a number of cancers, we aimed to identify miRNA biomarkers of breast cancer presence.
Circulating miRNAs were extracted from 200 µl plasma of 16 healthy females, 42 postmenopausal patients with ER+ primary breast cancer and 10 post-surgical breast cancer patients. MiRNAs were extracted using the Qiagen miRNeasy Serum/Plasma kit. Healthy controls were spilt into 2 pools, cancer samples were split into 4 pools and post-surgical samples were in 1 pool (8 - 10 samples in each), and each pool analysed using TaqMan Low-Density Array (pool A v2.1).
A two-tailed Spearmans analysis showed high correlation of the two healthy control pools, as well as high correlation between the 4 cancer pools (p<0.0001 for both). Analysis of 384 miRNAs gave a number of possibly candidates as biomarkers for breast cancer presence. Three miRNAs (hsa-miR-A, hsa-miR-B and hsa-miR-C) were absent in the two pools of healthy female controls, but were detected at significantly high levels in patients with ER+ breast cancer. Hsa-miR-C returned to base levels in the 10 post-surgical breast cancer patients, whereas hsa-miR-A, hsa-miR-B remained at significantly high levels.
The use of TLDA cards can be reproducibly performed, highlighting that circulating miRNAs are promising biomarkers for detection and monitoring of ER+ breast cancer. Validation in a larger series is ongoing in our group.