A6: Curcumin and its derivative as novel pharmacological treatment for human breast cancer cell line , invitro study
1Faculty of Medicine Mansoura University, Mansoura, Egypt,2Faculty of Pharmacy Mansoura University, Mansoura, Egypt,3Faculty of Science Mansoura University, Mansoura, Egypt,4Medical Experimental Research Center, Mansoura, Egypt
Curcumin, the principal curcuminoid found in turmeric, is generally considered its most active constituent . Other curcuminoids found in turmeric include demethoxycurcumin and bisdemethoxycurcumin. In addition to its use as a spice and pigment, turmeric has been used in India for medicinal purposes for centuries. More recently, evidence that curcumin may have anti-inflammatory and anticancer activities has renewed scientific interest in its potential to prevent and treat disease
The MCF-7 cells were cultured in DMEM .
The curcumins were dissolved in 100% dimethylsulfoxide
diluted to their final concentrations in serum free media
In all the experiments, the control cells were incubated with DMSO alone.
Cytotoxicity was assessed with the MTT assay
The experiments were repeated 3 times and data are expressed as means ± standard deviation (SD). This assay relies on the ability of metabolically active viable cells to reduce a yellow tetrazolium salt to a purple formazan product.
The cells were grown in 96 well plates .
Following incubation with the reagents, the medium was removed and the cells were treated with 75 ?l of MTT
(1 mg? ml) for 3 h at 37?C. Subsequently
, 100 ?l DMSO was added to each well. The solubilized formazan product was spectrophotometrically quantified using a PowerWave XS microplate reader
Anticancer activity was expressed as the concentration that caused 50% loss of cell monolayer (IC50 ).Using this approach,dose response and time course cytotoxicity of the standard agent ,5-FU, were initially carried out .The dose response effect of 5-fu was more evident at 72 hours of incubation than at 48 hours ,whereas at 24 hours IC50 was not converged with 5-fu
at any concentration
therefor 72 hours has been chosen as the incubation period for the dose-viability response of all tested compounds
Among all the tested compounds the following arrangement give the most active cytotoxic compounds against breast cancer MCF7 cell line
The most potent of these compounds are natural curcumin and 7a with IC50 values less than 10 ?M . Decreasing the 7 carbon spacer and substitution of (O-me) ,( O-Et), ( OH) or ( Cl) in R3 position decreased the activity of curcumin. Overall, this review concludes that curcumin and compound 7a show future potential as a powerful broad-spectrum treatment for breast cancer.