A18: GKT-AML5, WT1 and SSX2IP are frequently expressed in adult B-cell acute lymphocytic leukaemia

Payalben Savaliya1,Laurence Orchard2,Kim Orchard3,Barbara Guinn1

1University of Bedfordshire, Luton,Bedfordshire, UK,2Southampton University Hospital, Southampton, UK,3Southampton University Hospital, Southampton, UK,4University of Bedfordshire, Luton,Bedfordshire, UK

Presenting date: Monday 2 November
Presenting time: 13.10-14.00

Background

 

 

Acute lymphoblastic leukaemia (ALL) is a form of leukaemia characterized by excess lymphoblasts. If untreated the disease progresses rapidly and can be fatal within weeks to months. Adult patients with ALL who have an allogeneic stem cell transplant (allo-SCT) have an improved overall survival rate of 27-65% compared with 15-45% in patients receiving chemotherapy only (1-3).The benefits of allogeneic transplantation may in part be due to a ‘graft-versus-leukaemia’ response however in ALL few diseases related target antigens have been described. The identification of ‘ALL-associated antigens' has the potential to identify new targets for immunotherapy during first remission when tumour loads are low, or post-transplant when healthy donor T cells persist and the occurrence of graft versus leukaemia responses can crucially improve outcome.

 

Method

 

 

Using RT-PCR we have analysed the expression of 23 known tumour antigens, including the five identified by antibody specific-profiling (Bonney et al, in preparation). We examined their expression in leukaemia cell lines, presentation adult B-ALL patient samples and healthy volunteer peripheral blood mononuclear cell samples. All PCR products were sequenced to confirm their specificity, and that all PCRs were repeated at least three times.

 

Results

 

 

We found that the novel antigen, GKT-AML5, identified through the immunoscreening of an AML cDNA library with autologous sera (4), WT1 and SSX2IP are the most frequently expressed in patient samples but not found in healthy donor peripheral blood samples.

 

Conclusion

 

 

Further analysis of ALL patient samples and healthy donor leucocytes will indicate whether these antigens remain the most frequently expressed in adult B-ALL samples at disease diagnosis.