Imaging tumour metabolism using hyperpolarised 13C magnetic resonance spectroscopy
Session type: Symposia
Nuclear spin hyperpolarization techniques can increase sensitivity in the MR experiment by >10,000x. This has allowed us to image the location of labelled cell substrates and, more importantly, their metabolic conversion into other metabolites. These substrates include pyruvate , glutamine , glutamate , fumarate , bicarbonate  and ascorbate . We have shown that exchange of hyperpolarized 13C label between lactate and pyruvate can be imaged in animal models of lymphoma and glioma and that this flux is decreased post-treatment [1,7]. We showed that hyperpolarized [1,4-13C]fumarate can be used to detect tumour cell necrosis post treatment in lymphoma  and more recently that the polarized pyruvate and fumarate experiments can detect early evidence of treatment response in a breast tumour model  and can detect very early responses to anti-vascular  and anti-angiogenic drugs. We have shown that tissue pH can be imaged from the ratio of the signal intensities of hyperpolarized H13CO3- and 13CO2 following intravenous injection of hyperpolarized H13CO3¯  and that tumour redox state can be determined by monitoring the oxidation and reduction of [1-13C]ascorbate and [1-13C]dehydroascorbate respectively .
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