In utero low protein diet enhances estrogen exposure effects on adult rat prostate


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Jaqueline Rinaldi1, Carolina Sarobo1, Livia Lacorte1, Flavia Delella1, Andrei Moroz1, Andrelson Rinaldi2, Luis Justulin Jr1, Sergio Felisbino1
1Univ Estadual Paulista (UNESP), Botucatu, São Paulo, Brazil,2Universidade Federal da Grande Dourados (FACET), Faculdade de Ciências Exatas e Tecnológicas, Dourados, MS, Brazil

Background

Recent studies focusing the effect of fetal programming induced by in utero protein restriction on reproductive system demonstrated disorders in reproductive parameters, such as delay in sexual maturation and decrease in testicular weight in male rats. Moreover, perinatal plus adult estrogen exposures is related with development of prostatic diseases. Here, we investigate the effects of protein restriction fetal programming on rat prostate exposed chronically to low doses of estrogen and testosterone in the adult life.

Method

For this, 16 weeks-old wistar rats (n=48)  that received in utero normal diet (ND group, 17% protein) or underwent in utero protein restricted diet (PRD group, 6% protein) were subjected to 17-beta estradiol+testosterone administration (subcutaneous implant) for 16 weeks. Others animals from each group did not receive the implants. The animals were killed at age of 35 week and the ventral prostate (VP) and dorsolateral prostate (DLP) was excised, weighted and processed for histology. Analysis of prostatitis, metaplasia and neoplasias incidence were done.

Results

Both absolute and relative VP and DLP weight from ND and PRD animals without implants were not different. However, in the animal treated with estradiol and testosterone, the weight of VP and DLP from ND animals were higher than PRD (P<0.01). The prostates from PRD with implants also exhibited higher incidence of prostatitis, metaplasias  and neoplasias, than ND.

Conclusion

In conclusion, fetal programming induced by in utero protein restriction alters adult prostate response to androgen and estrogen handling and also interferes in prostate susceptibility to prostatitis, metaplasia and neoplasia.