A53: Lipids of peripheral blood mononuclear cells for cancer detection and evaluation

Yuri Tadevosyan1,Gohar Hakobyan1,Tigran Torgomyan1,Mihran Lazyan1,Hasmik Davtyan1,Rafik Ghazaryan1,Knarik Alexanyan2,Yelizaveta Amirkhanyan3

1Laboratory of Regulation of Cellular Activity, Institute of Molecular Biology, National Academy of Sciences of RA, Yerevan, Armenia,21Centre of Oncology after V.Fanarjyan, MH of RA, Yerevan, Armenia,3Centre of Hematology after R.Eolyan, MH of RA, Yerevan, Armenia

Presenting date: Monday 2 November
Presenting time: 12.20-13.10

Background

The involvement of cell plasma membrane (PM) lipids in the regulatory mechanisms of various important membrane-bound processes is well documented. It is known that compared to normal individuals, patients with several types of cancer have an increased prevalence of regulatory T cells (Treg) in the peripheral blood. The increase in Treg that suppresses autoimmunity may also inhibit the immune response against cancer. It was hypothesized by us earlier that in such intricate system diseases, as human tumors, alterations in lipid homeostasis in the PM of peripheral blood crude mononuclear cells (MNC) may possibly represent information characterizing the changes in anti- and pro-cancer potentials of blood that can be useful for detection and evaluation of malignancy.

Method

We investigated early (5 sec) and long-term (60 min) acylation of membrane lipids by exogenous [14C]arachidonic acid (AA) in MNC isolated from the peripheral blood of normal individuals and patients with diverse forms of leukemia and solid tumors. Alterations in the endogenous lipid second messengers (LSM) formation in [14C]AA-prelabelled MNC at different time points following T cell costimulation by anti-CD3/CD28 antibodies as well as in the activities of some endogenous lipid-metabolizing enzymes in the purified PM fraction of MNC were also investigated.

Results

The data obtained provide evidence for reproducible and identical defects in the processes of PM lipids fatty acid-content modification by AA, AA-1,2-diacyglycerol LSM molecule generation and membrane-associated enzyme activities in diverse forms of cancer. Importantly, alterations revealed were identical for all forms of malignancy and distinctly individual for each patient.

Conclusion

We conclude that the alterations observed are common characteristics of diverse cancers, and can be used for early detection and evaluation of malignancy as well as for discovery of new personalized treatment modes of disease.