Soluble CD44 in the serum and peritoneal fluid of the patients with Endometrial Cancer


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Zivar Salehi1,Sohail Mashayekhi2,Ebrahim Mirzajani3
1University of Guilan,2Faculty of Medicine, Guilan University of Medical Sciences, Rasht, Iran.,3Cellular and Molecular Research Center, Faculty of Medicine, Guilan University of Medical Sciences, Rasht, Iran.

Abstract

Background

CD44 is a membrane protein expressed by human endometrial cells, and it has been shown to promote the adhesion of endometrial cells. CD44 has been shown to bind to the cytoskeleton and membrane proteins. It was shown that CD44-hyaluronan interactions mediate cell adhesion and migration in a variety of physiological and pathophysiological processes including tumor metastasis.  Cell adhesion molecules exist in two forms including a membrane form, detectable in the cell surface, and a soluble form, detectable in biological fluids including serum and peritoneal fluid (PF). The soluble form of CD44 (sCD44), which consists of the ectodomain of CD44, has recently emerged as a key regulator of CD44 action. A variety of internal membrane receptors, including CD44, can be released from the lipid bilayer by proteolysis to form soluble, truncated proteins. The aim of this study was to analyze the concentrations of sCD44 in the serum and PF of patients with different stages of endometrial cancer (SC).

Method

A total of 132 peritoneal fluid and serum samples including 69 normal controls and 63 patients with different stages of SC were included in this study. The serum and peritoneal fluid concentration of sCD44 was measured by western blotting and enzyme linked immunosorbent assay (ELISA).

Results

The results showed that the sCD44 expression in serum and PF of patients with EC increased as compared to control group (P=0012). Moreover, an elevated level of sCD44 in serum and PF is associated with higher stages of EC.

Conclusion

Therefore, it is suggested that the measurement of serum or PF soluble c-Met may be useful in classifying EC. We conclude that sCD44 is always present in human serum and PF, and may be involved in the pathophysiology of endometrial cancer.