Strategic Bioassay guided fractionation – Isolation and characterization of novel, systematic, potent antiangiogenic molecules from seaweed associated actinomycetes


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Meenakshi Sundaram K1,Vincent S1,SUVRO Chatterjee2
1LOYOLA INSTITUTE OF FRONTIER ENERGY,2AUKBC, ANNA UNIVERSITY

Abstract

Background

The seaweeds are well-known for the presence of phytoestrogens that have promising anticancer activity. The actinomycetes are the popular source of bioactive secondary metabolites. A number of studies argued the need of pro-angiogenic based drug treatment containing the anticancer active metabolites are helping to create well-organized blood vessels that extent around the tumor mass for effective drug transportation. Such approach may ensure the cytotoxicity, anti-vascular and antiapoptotic activities for effective anticancer therapy. 

Results

We isolated five bacteria associated with seaweeds from Chennai, Tamil Nadu, Indian coastal region. Among them Actinomycetes sp., showed prominent bioactivity on neoangiogenesis (CAM assay). The study further evaluated the antimigration activity (Boydon chamber assay), antiinvasion and metastasis on non-invasive and invasive (HUVEC, MCF-7, MDA-MB-231, Bovine capillary endothelial cells (BCEC) and Aortic endothelial cells (AEC) cell lines using Bioassay guided fractionation for in vitro application using molecular level analysis.

Conclusion

Such type of systematic study will open an opportunity to find out the potential drug to understand the angiogenic molecular expression pattern from preliminary to in-depth knowledge for future drug discovery perspective.  Thus the antiangiogenic small molecules with novel structures could be a valuable tools for both deciphering the molecular mechanism of angiogenesis and developing new antiangiogenic therapeutic drugs.