The TFF1 promoter polymorphism and risk of gastric cancer


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Maryam Rasekhi1, Mehdi Moghanni bashi2, Parisa Mohamadynejad2, Mohammad Mohammadian Panah3, Abbas Ghaderi4, Saeed Khatamsaz2
1Islamic Azad university Arsanjan branch, Arsanjan/shiraz, Iran, Islamic Republic of,2Islamic Azad university Kazerun branch, kazerun/shiraz, Iran, Islamic Republic of,3Department of radiation oncology Namazi hospital Shiraz University of Medical sciences, Shiraz, Iran, Islamic Republic of,4Shiraz institute for cancer research Shiraz University of medical sciences, Shiraz, Iran, Islamic Republic of

Background

Mucosal damage is a key factor in the process of gastric cancer carcinogenesis. The trefoil factor family 1 (TFF1) protein, a member of trefoil factor family peptides, contributes to the protection of the gastrointestinal mucosa from injury by strengthening and stabilizing mucin gels, stimulating epithelial restitution, and restraining the associated inflammation. TFF1 expression is decreased in 50% of gastric cancer patients and defined as a tumor suppressor gene. In this context, the aim of our project is to study the role of TFF1 promoter single nucleotide polymorphisms (SNPs) in gastric cancer.

Method

To evaluate the role of TFF1 gene in gastric cancer risk, the TFF1 promoter region was amplified by PCR and sequenced in 50 cases and 50 controls matched for age and sex from Iranian population.

Results

We found one SNP in the promoter region of TFF1 gene in our population same as has been reported in NCBI database. Among cases, the distribution of genotypes was as follows: 54% were GG, 40% were GA, and 12% were AA. Among controls, the distribution was as follows: 64% were GG, 34% were GA, and 2% were AA. The A allele was found to be more common in case subjects than in control subjects (cases, 0.26; controls, 0.19) corresponding to a nominally significant increase in gastric cancer risk to heterozygotes and AA homozygotes (OR= 1.51, CI=0.67- 3.3) in the dominant inheritance model.

Conclusion

These results suggest that this SNP in TFF1 promoter affects genetic susceptibility to gastric cancer.